Journal: Open Life Sciences

Article Title: Sini Decoction Intervention on Atherosclerosis via PPARγ-LXRα-ABCA1 Pathway in Rabbits

doi: 10.1515/biol-2018-0053

Figure Lengend Snippet: Expression profiling of PPARγ-LXRα signaling to confirm the mechanism of treating AS by SND. The PPARγ (A-D) and LXRα (E-H) proteins levels in liver, peritoneal macrophages, PMC, and adipose tissue were measured by western blotting. The relative quantities were analyzed by ImageJ software. Data shown as mean ± SEM (n = 6). # P<0.05, compared with Chow group; *P<0.05, compared with HFD group.

Article Snippet: Primary antibodies to ABCA1 and Apo-B were purchased from Abcam (MA, USA); PPARγ antibody and LXRα antibody were purchased from Proteintech (IL, USA); and ApoA-I antibody was purchased from MyBioSource (CA, USA).

Techniques: Expressing, Western Blot, Software

Journal: Drug Design, Development and Therapy

Article Title: Asiatic acid protests against myocardial ischemia/reperfusion injury via modulation of glycometabolism in rat cardiomyocyte

doi: 10.2147/DDDT.S175116

Figure Lengend Snippet: Asiatic acid increases PPARγ levels and promotes GLUT4 translocation to plasma membrane in ischemic rats. Notes: The myocardium was isolated from vehicle-, asiatic acid (AA)-, and asiatic acid plus LY294002 (AA+LY)-treated rats under normal condition and following 1 hour of myocardial ischemia and 24 hours of reperfusion, respectively. The mRNA levels of ( A ) PPARγ and ( B ) GLUT4 were determined by real-time PCR. ( C ) PPARγ, GLUT4 from plasma membrane (PM GLUT4), GLUT4, and GAPDH levels were determined by Western blot. ( D ) Quantitative analysis of PPARγ levels were normalized to GAPDH levels, and ( E ) GLUT4 translocation levels (PM GLUT4) were normalized to total GLUT4. # P <0.05, ## P <0.01 vs the sham group; ** P <0.01 vs the vehicle group; && P <0.01 vs the asiatic acid-treated group. Abbreviation: MI/R, myocardial ischemia/reperfusion.

Article Snippet: The anti-Akt and anti-phosphor-Akt antibodies were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA); the anti-GSK-3β and anti-phospho-GSK-3β (Ser9) antibodies were from Cell Signaling Technology, Inc. (Danvers, MA, USA); the anti-GLUT4 and anti-PPARγ antibody was from Boster Biological Technology, Ltd. (Wuhan, China); and the anti-glyceraldehyde phosphate dehydrogenase (GAPDH) antibody was obtained from KangChen Bio-tech Inc. (Shanghai, China).

Techniques: Translocation Assay, Clinical Proteomics, Membrane, Isolation, Real-time Polymerase Chain Reaction, Western Blot

Journal: PLoS ONE

Article Title: Expression of Obesity Markers and Persistent Organic Pollutants Levels in Adipose Tissue of Obese Patients: Reinforcing the Obesogen Hypothesis?

doi: 10.1371/journal.pone.0084816

Figure Lengend Snippet: Data represent mean (±SE) of the serum concentrations of leptin and adiponectin (A) and gene expression of leptin, adiponectin, TNFα and PPARγ normalised to the gene expression of TBP gene*1000 of AT samples (B, C, D, E). Significant differences between men (n = 17) and women (n = 33) (B, C), diabetic (n = 8) and non diabetic patients (n = 42) (D, E) are indicated with asterisks (Mann Whitney U-test; *p≤0.05; **p≤0.01).

Article Snippet: Template standards and primers were obtained from OriGene Technologies (Rockville, MD) for every measured gene: PPARγ (HK210365), TNFα (HK208349), ADIPOQ (HK209573) and LEP (HK204316).

Techniques: Expressing, MANN-WHITNEY

Journal: eLife

Article Title: Bladder-cancer-associated mutations in RXRA activate peroxisome proliferator-activated receptors to drive urothelial proliferation

doi: 10.7554/eLife.30862

Figure Lengend Snippet:

Article Snippet: recombinant DNA reagent , pCMV6-XL4 PPARG (plasmid) , OriGene , OriGene:SC108192 , .

Techniques: Generated, Plasmid Preparation, Infection, In Vitro, Recombinant, Mutagenesis, Clone Assay, Sequencing, Luciferase, Software